The Three Stooges attempted pathos in Cash and Carry (1937). The Stooges arrive at their home, a dilapidated shack in the city dump, only to find a little boy they don’t know doing his homework at the kitchen table. The Stooges do not react with great sentiment to this child. Larry pipes up, “Come on, beat it.” But, then, they realize that the boy is wearing a leg brace and needs the support of a crutch to stand and walk. The boy tells the Stooges that his mom and dad are gone and he’s being raised by his big sister. Moe, normally gruff and scowling, smiles sympathetically and softens his voice. He has never acted more tenderly to another individual. But the other two Stooges do not show much concern. […] Seconds after meeting the crippled orphan boy, Curly accidentally hits Moe in the head with a pipe and it’s back to their usual comedy business. A pathos comedy was, in the end, no more significant to the Stooges than a haunted house comedy.
From Why You Want to Bring Me Down?, a great comparison of the use (and perhaps more often, misuse) of pathos in comedies, from Charlie Chaplin and Harold Lloyd to Adam Sandler and Robin Williams.